Faculty Profile - David Karl BANFIELD | The Hong Kong University of Science and Technology

David Karl BANFIELD
彭大衛

PhD in Biochemistry
The University of British Columbia, 1991

Professor
Division of Life Science

ORCID

0000-0002-5492-5741

Scopus ID

56458194600

Research Interest

Intracellular protein trafficking
Secretory organelle maintenance

Publications

2024 1

SM protein Sly1 and a SNARE Habc domain promote membrane fusion through multiple mechanisms

Journal of Cell Biology, v. 223, (6), March 2024, article number e202001034, p. 1-23
Duan, Mengtong; Gao, Guanbin; Lin, Ariel; Mackey, Emma J.; Banfield, David Karl; Merz, Alexey J
Article

2022 2

Engineering yeast to induce the synthesis of GPI-APs with a permanent phosphoethanolamine on mannose 2 of the glycan moiety

STAR Protocols, v. 3, (3), 16 September 2022, article number 101503
Chen, Li; Banfield, David Karl
Article

The flexible N-terminal motif of uL11 unique to eukaryotic ribosomes interacts with P-complex and facilitates protein translation

NUCLEIC ACIDS RESEARCH, v. 50, (9), 20 May 2022, p. 5335-5348
Yang, Lei; Lee, Ka-Ming; Yu, Conny Wing-Heng; Imai, Hirotatsu; Choi, Andrew Kwok-Ho; Banfield, David Karl; Ito, Kosuke; Uchiumi, Toshio; Wong, Kam-Bo
Article

2021 1

Remodeling-defective GPI-anchored proteins on the plasma membrane activate the spindle assembly checkpoint

Cell Reports, v. 37, (13), December 2021, article number 110120
Chen, Li; Tu, Linna; Yang, Gege; Banfield, David Karl
Article

2020 3

A novel mechanism for the retention of Golgi membrane proteins mediated by the Bre5p/Ubp3p deubiquitinase complex

Molecular Biology of the Cell, v. 31, (19), September 2020, p. 2139-2155
Wang, Peng; Ye, Ziyun; Banfield, David K
Article

Cdc1p is a Golgi-localized glycosylphosphatidylinositol-anchored protein remodelase

Molecular Biology of the Cell, v. 31, (26), December 2020, p. 2883-2891
Yang, Gege; Banfield, David Karl
Article

Multiple features within the syntaxin Sed5p mediate its Golgi localization

Traffic, v. 21, (3), March 2020, p. 274-296
Gao, Guanbin; Banfield, David Karl
Article

2019 1

Structural and Mutagenesis Studies Evince the Role of the Extended Protuberant Domain of Ribosomal Protein uL10 in Protein Translation

Biochemistry, v. 58, (36), September 2019, p. 3744-3754
Choi, Kwok-Ho Andrew; Yang, Lei; Lee, Ka-Ming; Yu, Conny Wing-Heng; Banfield, David Karl; Ito, Kosuke; Uchiumi, Toshio; Wong, Kam-Bo
Article

2016 1

Multiple ER-Golgi SNARE Transmembrane domains are Dispensable for Trafficking but Required for SNARE Recycling

Molecular Biology of the Cell, v. 27, (17), September 2016, p. 2633-2641
Chen, Li; Lau, Martin S. Y.; Banfield, David Karl
Article

2015 1

Conserved function of the lysine-based KXD/E motif in Golgi retention for endomembrane proteins among different organisms

Molecular Biology of the Cell, v. 26, (23), November 2015, p. 4280-4293
Woo, Cheukhang; Gao, Caiji; Yu, Ping; Tu, Linna; Meng, Zhaoyue; Banfield, David Karl; Yao, Xiaoqiang; Jiang, Liwen
Article

2014 1

Cell Organelles

Banfield, David K.; Prinz, Will
Book

2012 1

A Conserved N-terminal Arginine-Motif in GOLPH3-Family Proteins Mediates Binding to Coatomer

Traffic, v. 13, (11), November 2012, p. 1496-1507
Tu, Linna; Chen, Lu; Banfield, David K.
Article

2011 1

Mechanisms of Protein Retention in the Golgi

Cold Spring Harbor Perspectives in Biology, v. 3, (8), August 2011, p. 1-14
Banfield, D.K.
Article

2010 2

Localization of Golgi-resident Glycosyltransferases

Cellular and molecular life sciences, v. 67, (1), 2010, JAN, p. 29-41
Tu, Linna; Banfield, David Karl
Article

Signal-mediated retention of Golgi-resident integral membrane proteins

Second International Symposium on Membrane Biology, Ningbo, China, 2010
Banfield, David
Conference paper

2009 2

Identification of the Sub-unit Interactions and Sorting Signals that Mediate COPI-dependent Trafficking of Yeast SNAREs

2009 Gordon Research Conference on Molecular Membrane Biology, Proctor Academy, Andover, New Hampshire (USA), 5-10 Jul 2009
Banfield, David
Conference paper

Localization of Glycosyltransferases in the Golgi.

2009 IUBMB Congress, Shanghai, PRC China (August, 2009), Shanghai, PRC China
Banfield, David K.
Conference paper

2008 5

Signal-Mediated Dynamic Retention of Glycosyltransferases in the Golgi

Science, v. 321, (5887), 2008, JUL 18, p. 404-407
Tu, Linna; Tai, William C.S.; Chen, Lu; Banfield, David K.
Article

The Golgi and ER syntaxins control anterograde and retrograde transport through the early secretory pathway

Traffic, Volume10, Pages 1629-1652
Bubeck, J.; Langhans, M.; Scheuring, D.; Foresti, O.; Banfiled, David; Denecke, J.; Robinson, D.
Article

The Syntaxins SYP31 and SYP81 Control ER–Golgi Trafficking in the Plant Secretory Pathway

Traffic, v. 9, (10), 2008, OCT, p. 1629-1652
Bubeck, Julia; Scheuring, David; Hummel, Eric; Langhans, Markus; Viotti, Corrado; Foresti, Ombretta; Denecke, Juergen; Banfield, David K.; Robinson, David G.
Article

SNAREs

The Golgi apparatus - the state of the art 110 years after Camillo's Discovery / Edited by A. Mironov and M. Pavelka. Wien : Springer, 2008, p. 43-65
Banfield, David; Hong, Wanjin
Book chapter

Characterization of a Novel Longin Domain Containing Protein from Yeast

Hunter Cellular Biology Meeting, Pokolbin, NSW, Australia
Banfield, David
Conference paper

2007 1

Signal Mediated Retention of Glycosyltransferases in the Yeast Golgi

Gordon Research Conference on Molecular Membrane Biology, Andover, New Hampshire
Banfield, David
Conference paper

2006 2

Identification of the yeast R-SNARE Nyv1p as a novel longin domain-containing protein

Molecular biology of the cell, v. 17, (10), 2006, OCT, p. 4282-4299
Wen, Wenyu; Chen, Lu; Wu, Hao; Sun, Xin; Zhang, Mingjie; Banfield, David K.
Article

Identification of the Yeast R-SNARE Nyv1p as a Novel Longin Domain-Containing Protein

46th Annual Meeting of the American Society for Cell Biology, San Diego Convention center, San Diego, California (USA), 9-13 Dec 2006
Chen, Lu; Wen, Wenyu; Wu, Hao; Sun, Xin; Zhang, Mingjie; Banfield, David K.
Conference paper

2004 4

A Novel Regulator of COPI-coated Vesicle Formation

British Society for Cell Biology Annual Spring Meeting, University of Kent at Canterbury, 31 March-3 April
Tai, William C.S.; Banfield, David K.
Conference paper

COPI-coated Vesicle Formation and SNAREs

Keystone Symposia: Golgi Apparatus and Secretory Pathway of Eukaryotic Cells, Breckenridge Colorado, USA, 20-25, January
Tai, William C.S.; Banfield, David K.
Conference paper

The Molecular Architecture of Yeast ER / Golgi SNAREs.

48th Annual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology, Banff. Alberta, Canada, March 16-20, 2005
Li, Wai Ming; Tsui, Macro; Banfield, David
Conference paper

The R-SNARE Nyv1p is sorted to the limiting membrane of the yeast cell vacuole via an AP-3-dependent YXXF signal in its longin domain.

48th Annual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology, Banff. Alberta, Canada, March 16-20, 2005
Chen, Lu; Wen, Wenyu; Wu, Hao; Zhang, Mingjie; Banfield, David K.
Conference paper

2002 1

Genes that Allow Cells to Grow in the Absence of the Golgi SNARE Sft1p: Identification of New Components of the Vesicle Budding Machinery.

European Science Foundation meeting 'EXOCYTOSIS: Membrane structure and dynamics.'
Tai, William; Banfield, David Karl
Conference paper

2001 5

An autoinhibitory mechanism for nonsyntaxin SNARE proteins revealed by the structure of Ykt6p

Science, v. 293, (5530), 2001, JUL 27, p. 698-702
Tochio, Hidehito; Tsui, M.M.K.; Banfield, David K.; Zhang, Mingjie
Article

AtBS14a and AtBS14b, two Bet1/Sft1-like SNAREs from Arabidopsis thaliana that complement mutations in the yeast SFT1 gene

FEBS letters, v. 500, (3), 2001, JUL 6, p. 177-182
Tai, WCS; Banfield, DK
Article

Selective formation of Sed5p-containing SNARE complexes is mediated by combinatorial binding interactions

Molecular biology of the cell, v. 12, (3), 2001, MAR, p. 521-538
Tsui, MMK; Tai, WCS; Banfield, DK
Article

SNARE complexes - Is there sufficient complexity for vesicle targeting specificity?

Trends in Biochemical Sciences, v. 26, (1), 2001, p. 67-68
Banfield, D.K.
Article

An Auto-Inhibitory Mechanism for Non-Syntaxin SNARE Proteins Revealed by the Structure of Ykt6p.

the Cold Spring Harbor Yeast Cell Biology Meeting
Banfield, David K.; Tochio, Hidehito; Tsui, Marco; Zhang, Mingjie
Conference paper

2000 1

Yeast Golgi SNARE interactions are promiscuous

Journal of cell science, v. 113, (1), 2000, JAN, p. 145-152
Tsui, MMK; Banfield, DK
Article

1995 2

A SNARE-like Protein Required for Traffic through the Golgi Complex

Nature, v. 375, 1995, p. 806-809
Lewis, M.J.; Pelham, H.R.B.; Banfield, David K.
Article

SNAREs involved in traffic through the Golgi Complex

Cold Spring Harbor Symposia on Quantitative Biology, v. 60, 1995, p. 105-111
Pelham, H.R.B.; Banfield, David K.; Lewis, M.J.
Article

1994 3

Characterization of a Stable Form of Human Meizothrombin Derived from Recombinant Prothrombin (R155A, R271A, R284A)

Journal of Biological Chemistry, v. 269, 1994, p. 11374-11380
Côté, H.C.F.; Stevens, W.K.; Bajzar, B.; Banfield, David K.; Nesheim, M.E.; MacGillivray, R.T.A.
Article

Evolution of Prothrombin: Isolation and Characterization of the cDNAs Encoding Chicken and Hagfish Prothrombin

Journal of Molecular Evolution, v. 38, 1994, p. 177-187
Irwin, D.M.; Walz, D.A.; MacGillivray, R.T.A.; Banfield, David K.
Article

Localisation of Sed5, a Putative Vesicle Targeting Molecule, to the cis-Golgi Network Involves both its Transmembrane and Cytoplasmic Domains

Journal of Cell Biology, v. 127, 1994, p. 357-371
Lewis, M.J.; Rabouille, C.; Warren, G.; Pelham, H.R.B.; Banfield, David K.
Article

1993 3

Characterization of Wild-Type and an Active-Site Mutant in Escherichia coli of Short-Chain Acyl-CoA Dehydrogenase from Megasphaera elsdenii

Biochemistry, v. 32, 1993, p. 10736-10742
Becker, D.F.; Fuchs, J.A.; Banfield, David K.; Funk, W.; MacGillivray, R.T.A.; Stankovich, M.T.
Article

Developmental Expression of Chicken Antithrombin III is Regulated by Increased RNA Abundance and Intracellular Processing

Biochimica et Biophysica Acta, v. 1171, 1993, p. 239-246
Amrani, D.L.; Rosenberg, J.; Samad, F.; Bergtrom, G.; Banfield, David K.
Article

Expression of Glycosylated and Non-glycosylated Human Transferrin in Mammalian Cells

Initial Characterization of the Recombinant Proteins with Comparison to Three Commercially Available Transferrin. Biochemistry, v. 32, 1993, p. 5472-5479
Mason, A.B.; Miller, M.K.; Funk, W.D.; Banfield, David K.; Savage, K.S.; Oliver, R.W.A.; Green, B.N.; MacGillivray, R.T.A.; Woodworth, R.C.
Article

1992 2

Partial Characterization of Vertebrate Prothrombin cDNAs: Amplification and Sequence Analysis of the B-Chain of Thrombin from Nine Different Species

Proceedings of the National Academy of Sciences, v. 89, 1992, p. 2779-2783
Banfield, David K.; MacGillivray, R.T.A.
Article

The Isolation and Characterization of Rabbit Motilin Precursor cDNA

Biochimica et Biophysica Acta, v. 1131, 1992, p. 341-344
MacGillivray, R.T.A.; Brown, J.C.; McIntosh, C.H.S.; Banfield, David K.
Article

1991 5

Apolipoprotein E Genotyping using the Polymerase Chain Reaction and Allele Specific Primers

Journal of Lipid Research, v. 32, 1991, p. 183-187
Main, B.F.; Jones, P.J.H.; MacGillivray, R.T.A.; Banfield, David K.
Article

Nucleotide Sequence of Rabbit Liver Transferrin cDNA

Biochimica et Biophysica Acta, v. 1089, 1991, p. 262-265
Chow, B. KC; Funk, W.; Robertson, K.; Umelas, T.; Banfield, David K.; Woodworth, R.C.; MacGillivray, R.T.A.
Article

Rapid Apolipoprotein E Genotype Identification

PCR Topics: usage of the polymerase chain reaction in genetic and infectious diseases, 1991, p. 99-102
Main, B.F.; Jones, P.J.H.; MacGillivray, R.T.A.; Banfield, David K.
Article

Structural-Functional Studies of Human Transferrin by Using In Vitro Mutagenesis

Current Studies in Heamatology and Blood Transfusion, v. 58, 1991, p. 132-138
Chow, B. KC; Funk, W.; Banfield, David K.; Lineback, J.A.; Mason, A.B.; Woodworth, R.C.; MacGillivray, R.T.A.
Article

Rapid Apolipoprotein E Genotype Identification in PCR Topics

Usage of the polymerase chain reaction in genetic and infectious diseases / Edited by Rolfs, A., Schumacher, H., and Marx, P., Berlin, Springer-Verlag, 1991, p. 99-102
Main, B.F.; Jones, P.J.H.; MacGillivray, R.T.A.; Banfield, David K.
Book chapter

1990 3

Apolipoprotein E genotyping using the polymerase chain reaction

New Zealand Medical Journal, v. 103, (884) 1990, p. 80-81
Main, Blair F.; Banfield, David K.; Jones, Peter Jh Harris
Article

Nucleotide sequence of nine protein-coding genes and 22 tRNAs in the mitochondrial DNA of the sea star Pisaster ochraceus

Institute of Molecular Biology and Biochemistry, Department of Biological Sciences, Simon Fraser University, Burnaby, V5A 1S6, British Columbia, Canada, v.31, (3), 1990, 195-204
Banfield, David K.; Smith, Michael J.; Doteval, Karin; Gorski, Sharon M.; Kowbel, David J.
Article

Protein Coding Sequences in the Sea Star Mitochondrial Genome

Journal of Molecular Evolution, v. 31, 1990, p. 195-204
Smith, M.J.; Banfield, David K.; Doteval, K.; Gorski, S.; Kowbel, D.J.
Article

1989 1

Gene Arrangement in Sea Star Mitochondrial DNA Demonstrates a Major Inversion Event During Echinoderm Evolution

Gene, v. 76, p. 181-185
Smith, M.J.; Banfield, David K.; Doteval, K.; Gorski, S.; Kowbel, D.J.
Article

1988 2

H3 Histone RNA in Eggs and Embryos of the Sea Star Piaster ochraceus

Biochemistry and Cell Biology, v. 66, 1988, p. 1040-1044
Banfield, David K.
Article

Histone Genes in Three Sea Star Species: Cluster Arrangement, Transcriptional Polarity, and Analysis of the Flanking Regions of H3 and H4 Genes

Journal of Molecular Evolution, v. 27, 1988, p. 36-44
Cool, D.; Banfield, David K.; Honda, B.M.; Smith, M.J.
Article

Article 1

SM protein Sly1 and a SNARE Habc domain promote membrane fusion through multiple mechanisms

Journal of Cell Biology, v. 223, (6), March 2024, article number e202001034, p. 1-23
Duan, Mengtong; Gao, Guanbin; Lin, Ariel; Mackey, Emma J.; Banfield, David Karl; Merz, Alexey J

Article 2

Engineering yeast to induce the synthesis of GPI-APs with a permanent phosphoethanolamine on mannose 2 of the glycan moiety

STAR Protocols, v. 3, (3), 16 September 2022, article number 101503
Chen, Li; Banfield, David Karl

The flexible N-terminal motif of uL11 unique to eukaryotic ribosomes interacts with P-complex and facilitates protein translation

NUCLEIC ACIDS RESEARCH, v. 50, (9), 20 May 2022, p. 5335-5348
Yang, Lei; Lee, Ka-Ming; Yu, Conny Wing-Heng; Imai, Hirotatsu; Choi, Andrew Kwok-Ho; Banfield, David Karl; Ito, Kosuke; Uchiumi, Toshio; Wong, Kam-Bo

Article 1

Remodeling-defective GPI-anchored proteins on the plasma membrane activate the spindle assembly checkpoint

Cell Reports, v. 37, (13), December 2021, article number 110120
Chen, Li; Tu, Linna; Yang, Gege; Banfield, David Karl

Article 3

A novel mechanism for the retention of Golgi membrane proteins mediated by the Bre5p/Ubp3p deubiquitinase complex

Molecular Biology of the Cell, v. 31, (19), September 2020, p. 2139-2155
Wang, Peng; Ye, Ziyun; Banfield, David K

Cdc1p is a Golgi-localized glycosylphosphatidylinositol-anchored protein remodelase

Molecular Biology of the Cell, v. 31, (26), December 2020, p. 2883-2891
Yang, Gege; Banfield, David Karl

Multiple features within the syntaxin Sed5p mediate its Golgi localization

Traffic, v. 21, (3), March 2020, p. 274-296
Gao, Guanbin; Banfield, David Karl

Article 1

Structural and Mutagenesis Studies Evince the Role of the Extended Protuberant Domain of Ribosomal Protein uL10 in Protein Translation

Biochemistry, v. 58, (36), September 2019, p. 3744-3754
Choi, Kwok-Ho Andrew; Yang, Lei; Lee, Ka-Ming; Yu, Conny Wing-Heng; Banfield, David Karl; Ito, Kosuke; Uchiumi, Toshio; Wong, Kam-Bo

Article 1

Multiple ER-Golgi SNARE Transmembrane domains are Dispensable for Trafficking but Required for SNARE Recycling

Molecular Biology of the Cell, v. 27, (17), September 2016, p. 2633-2641
Chen, Li; Lau, Martin S. Y.; Banfield, David Karl

Article 1

Conserved function of the lysine-based KXD/E motif in Golgi retention for endomembrane proteins among different organisms

Molecular Biology of the Cell, v. 26, (23), November 2015, p. 4280-4293
Woo, Cheukhang; Gao, Caiji; Yu, Ping; Tu, Linna; Meng, Zhaoyue; Banfield, David Karl; Yao, Xiaoqiang; Jiang, Liwen

Book 1

Cell Organelles

Banfield, David K.; Prinz, Will

Article 1

A Conserved N-terminal Arginine-Motif in GOLPH3-Family Proteins Mediates Binding to Coatomer

Traffic, v. 13, (11), November 2012, p. 1496-1507
Tu, Linna; Chen, Lu; Banfield, David K.

Article 1

Mechanisms of Protein Retention in the Golgi

Cold Spring Harbor Perspectives in Biology, v. 3, (8), August 2011, p. 1-14
Banfield, D.K.

Article 1

Localization of Golgi-resident Glycosyltransferases

Cellular and molecular life sciences, v. 67, (1), 2010, JAN, p. 29-41
Tu, Linna; Banfield, David Karl

Conference paper 1

Signal-mediated retention of Golgi-resident integral membrane proteins

Second International Symposium on Membrane Biology, Ningbo, China, 2010
Banfield, David

Conference paper 2

Identification of the Sub-unit Interactions and Sorting Signals that Mediate COPI-dependent Trafficking of Yeast SNAREs

2009 Gordon Research Conference on Molecular Membrane Biology, Proctor Academy, Andover, New Hampshire (USA), 5-10 Jul 2009
Banfield, David

Localization of Glycosyltransferases in the Golgi.

2009 IUBMB Congress, Shanghai, PRC China (August, 2009), Shanghai, PRC China
Banfield, David K.

Article 3

Signal-Mediated Dynamic Retention of Glycosyltransferases in the Golgi

Science, v. 321, (5887), 2008, JUL 18, p. 404-407
Tu, Linna; Tai, William C.S.; Chen, Lu; Banfield, David K.

The Golgi and ER syntaxins control anterograde and retrograde transport through the early secretory pathway

Traffic, Volume10, Pages 1629-1652
Bubeck, J.; Langhans, M.; Scheuring, D.; Foresti, O.; Banfiled, David; Denecke, J.; Robinson, D.

The Syntaxins SYP31 and SYP81 Control ER–Golgi Trafficking in the Plant Secretory Pathway

Traffic, v. 9, (10), 2008, OCT, p. 1629-1652
Bubeck, Julia; Scheuring, David; Hummel, Eric; Langhans, Markus; Viotti, Corrado; Foresti, Ombretta; Denecke, Juergen; Banfield, David K.; Robinson, David G.

Book chapter 1

SNAREs

The Golgi apparatus - the state of the art 110 years after Camillo's Discovery / Edited by A. Mironov and M. Pavelka. Wien : Springer, 2008, p. 43-65
Banfield, David; Hong, Wanjin

Conference paper 1

Characterization of a Novel Longin Domain Containing Protein from Yeast

Hunter Cellular Biology Meeting, Pokolbin, NSW, Australia
Banfield, David

Conference paper 1

Signal Mediated Retention of Glycosyltransferases in the Yeast Golgi

Gordon Research Conference on Molecular Membrane Biology, Andover, New Hampshire
Banfield, David

Article 1

Identification of the yeast R-SNARE Nyv1p as a novel longin domain-containing protein

Molecular biology of the cell, v. 17, (10), 2006, OCT, p. 4282-4299
Wen, Wenyu; Chen, Lu; Wu, Hao; Sun, Xin; Zhang, Mingjie; Banfield, David K.

Conference paper 1

Identification of the Yeast R-SNARE Nyv1p as a Novel Longin Domain-Containing Protein

46th Annual Meeting of the American Society for Cell Biology, San Diego Convention center, San Diego, California (USA), 9-13 Dec 2006
Chen, Lu; Wen, Wenyu; Wu, Hao; Sun, Xin; Zhang, Mingjie; Banfield, David K.

Conference paper 4

A Novel Regulator of COPI-coated Vesicle Formation

British Society for Cell Biology Annual Spring Meeting, University of Kent at Canterbury, 31 March-3 April
Tai, William C.S.; Banfield, David K.

COPI-coated Vesicle Formation and SNAREs

Keystone Symposia: Golgi Apparatus and Secretory Pathway of Eukaryotic Cells, Breckenridge Colorado, USA, 20-25, January
Tai, William C.S.; Banfield, David K.

The Molecular Architecture of Yeast ER / Golgi SNAREs.

48th Annual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology, Banff. Alberta, Canada, March 16-20, 2005
Li, Wai Ming; Tsui, Macro; Banfield, David

The R-SNARE Nyv1p is sorted to the limiting membrane of the yeast cell vacuole via an AP-3-dependent YXXF signal in its longin domain.

48th Annual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology, Banff. Alberta, Canada, March 16-20, 2005
Chen, Lu; Wen, Wenyu; Wu, Hao; Zhang, Mingjie; Banfield, David K.

Conference paper 1

Genes that Allow Cells to Grow in the Absence of the Golgi SNARE Sft1p: Identification of New Components of the Vesicle Budding Machinery.

European Science Foundation meeting 'EXOCYTOSIS: Membrane structure and dynamics.'
Tai, William; Banfield, David Karl

Article 4

An autoinhibitory mechanism for nonsyntaxin SNARE proteins revealed by the structure of Ykt6p

Science, v. 293, (5530), 2001, JUL 27, p. 698-702
Tochio, Hidehito; Tsui, M.M.K.; Banfield, David K.; Zhang, Mingjie

AtBS14a and AtBS14b, two Bet1/Sft1-like SNAREs from Arabidopsis thaliana that complement mutations in the yeast SFT1 gene

FEBS letters, v. 500, (3), 2001, JUL 6, p. 177-182
Tai, WCS; Banfield, DK

Selective formation of Sed5p-containing SNARE complexes is mediated by combinatorial binding interactions

Molecular biology of the cell, v. 12, (3), 2001, MAR, p. 521-538
Tsui, MMK; Tai, WCS; Banfield, DK

SNARE complexes - Is there sufficient complexity for vesicle targeting specificity?

Trends in Biochemical Sciences, v. 26, (1), 2001, p. 67-68
Banfield, D.K.

Conference paper 1

An Auto-Inhibitory Mechanism for Non-Syntaxin SNARE Proteins Revealed by the Structure of Ykt6p.

the Cold Spring Harbor Yeast Cell Biology Meeting
Banfield, David K.; Tochio, Hidehito; Tsui, Marco; Zhang, Mingjie

Article 1

Yeast Golgi SNARE interactions are promiscuous

Journal of cell science, v. 113, (1), 2000, JAN, p. 145-152
Tsui, MMK; Banfield, DK

Article 2

A SNARE-like Protein Required for Traffic through the Golgi Complex

Nature, v. 375, 1995, p. 806-809
Lewis, M.J.; Pelham, H.R.B.; Banfield, David K.

SNAREs involved in traffic through the Golgi Complex

Cold Spring Harbor Symposia on Quantitative Biology, v. 60, 1995, p. 105-111
Pelham, H.R.B.; Banfield, David K.; Lewis, M.J.

Article 3

Characterization of a Stable Form of Human Meizothrombin Derived from Recombinant Prothrombin (R155A, R271A, R284A)

Journal of Biological Chemistry, v. 269, 1994, p. 11374-11380
Côté, H.C.F.; Stevens, W.K.; Bajzar, B.; Banfield, David K.; Nesheim, M.E.; MacGillivray, R.T.A.

Evolution of Prothrombin: Isolation and Characterization of the cDNAs Encoding Chicken and Hagfish Prothrombin

Journal of Molecular Evolution, v. 38, 1994, p. 177-187
Irwin, D.M.; Walz, D.A.; MacGillivray, R.T.A.; Banfield, David K.

Localisation of Sed5, a Putative Vesicle Targeting Molecule, to the cis-Golgi Network Involves both its Transmembrane and Cytoplasmic Domains

Journal of Cell Biology, v. 127, 1994, p. 357-371
Lewis, M.J.; Rabouille, C.; Warren, G.; Pelham, H.R.B.; Banfield, David K.

Article 3

Characterization of Wild-Type and an Active-Site Mutant in Escherichia coli of Short-Chain Acyl-CoA Dehydrogenase from Megasphaera elsdenii

Biochemistry, v. 32, 1993, p. 10736-10742
Becker, D.F.; Fuchs, J.A.; Banfield, David K.; Funk, W.; MacGillivray, R.T.A.; Stankovich, M.T.

Developmental Expression of Chicken Antithrombin III is Regulated by Increased RNA Abundance and Intracellular Processing

Biochimica et Biophysica Acta, v. 1171, 1993, p. 239-246
Amrani, D.L.; Rosenberg, J.; Samad, F.; Bergtrom, G.; Banfield, David K.

Expression of Glycosylated and Non-glycosylated Human Transferrin in Mammalian Cells

Initial Characterization of the Recombinant Proteins with Comparison to Three Commercially Available Transferrin. Biochemistry, v. 32, 1993, p. 5472-5479
Mason, A.B.; Miller, M.K.; Funk, W.D.; Banfield, David K.; Savage, K.S.; Oliver, R.W.A.; Green, B.N.; MacGillivray, R.T.A.; Woodworth, R.C.

Article 2

Partial Characterization of Vertebrate Prothrombin cDNAs: Amplification and Sequence Analysis of the B-Chain of Thrombin from Nine Different Species

Proceedings of the National Academy of Sciences, v. 89, 1992, p. 2779-2783
Banfield, David K.; MacGillivray, R.T.A.

The Isolation and Characterization of Rabbit Motilin Precursor cDNA

Biochimica et Biophysica Acta, v. 1131, 1992, p. 341-344
MacGillivray, R.T.A.; Brown, J.C.; McIntosh, C.H.S.; Banfield, David K.

Article 4

Apolipoprotein E Genotyping using the Polymerase Chain Reaction and Allele Specific Primers

Journal of Lipid Research, v. 32, 1991, p. 183-187
Main, B.F.; Jones, P.J.H.; MacGillivray, R.T.A.; Banfield, David K.

Nucleotide Sequence of Rabbit Liver Transferrin cDNA

Biochimica et Biophysica Acta, v. 1089, 1991, p. 262-265
Chow, B. KC; Funk, W.; Robertson, K.; Umelas, T.; Banfield, David K.; Woodworth, R.C.; MacGillivray, R.T.A.

Rapid Apolipoprotein E Genotype Identification

PCR Topics: usage of the polymerase chain reaction in genetic and infectious diseases, 1991, p. 99-102
Main, B.F.; Jones, P.J.H.; MacGillivray, R.T.A.; Banfield, David K.

Structural-Functional Studies of Human Transferrin by Using In Vitro Mutagenesis

Current Studies in Heamatology and Blood Transfusion, v. 58, 1991, p. 132-138
Chow, B. KC; Funk, W.; Banfield, David K.; Lineback, J.A.; Mason, A.B.; Woodworth, R.C.; MacGillivray, R.T.A.

Book chapter 1

Rapid Apolipoprotein E Genotype Identification in PCR Topics

Usage of the polymerase chain reaction in genetic and infectious diseases / Edited by Rolfs, A., Schumacher, H., and Marx, P., Berlin, Springer-Verlag, 1991, p. 99-102
Main, B.F.; Jones, P.J.H.; MacGillivray, R.T.A.; Banfield, David K.

Article 3

Apolipoprotein E genotyping using the polymerase chain reaction

New Zealand Medical Journal, v. 103, (884) 1990, p. 80-81
Main, Blair F.; Banfield, David K.; Jones, Peter Jh Harris

Nucleotide sequence of nine protein-coding genes and 22 tRNAs in the mitochondrial DNA of the sea star Pisaster ochraceus

Institute of Molecular Biology and Biochemistry, Department of Biological Sciences, Simon Fraser University, Burnaby, V5A 1S6, British Columbia, Canada, v.31, (3), 1990, 195-204
Banfield, David K.; Smith, Michael J.; Doteval, Karin; Gorski, Sharon M.; Kowbel, David J.

Protein Coding Sequences in the Sea Star Mitochondrial Genome

Journal of Molecular Evolution, v. 31, 1990, p. 195-204
Smith, M.J.; Banfield, David K.; Doteval, K.; Gorski, S.; Kowbel, D.J.

Article 1

Gene Arrangement in Sea Star Mitochondrial DNA Demonstrates a Major Inversion Event During Echinoderm Evolution

Gene, v. 76, p. 181-185
Smith, M.J.; Banfield, David K.; Doteval, K.; Gorski, S.; Kowbel, D.J.

Article 2

H3 Histone RNA in Eggs and Embryos of the Sea Star Piaster ochraceus

Biochemistry and Cell Biology, v. 66, 1988, p. 1040-1044
Banfield, David K.

Histone Genes in Three Sea Star Species: Cluster Arrangement, Transcriptional Polarity, and Analysis of the Flanking Regions of H3 and H4 Genes

Journal of Molecular Evolution, v. 27, 1988, p. 36-44
Cool, D.; Banfield, David K.; Honda, B.M.; Smith, M.J.

2019 1

Structural and Mutagenesis Studies Evince the Role of the Extended Protuberant Domain of Ribosomal Protein uL10 in Protein Translation

Biochemistry, v. 58, (36), September 2019, p. 3744-3754
Choi, Kwok-Ho Andrew; Yang, Lei; Lee, Ka-Ming; Yu, Conny Wing-Heng; Banfield, David Karl; Ito, Kosuke; Uchiumi, Toshio; Wong, Kam-Bo
Article

2016 1

Multiple ER-Golgi SNARE Transmembrane domains are Dispensable for Trafficking but Required for SNARE Recycling

Molecular Biology of the Cell, v. 27, (17), September 2016, p. 2633-2641
Chen, Li; Lau, Martin S. Y.; Banfield, David Karl
Article

2015 1

Conserved function of the lysine-based KXD/E motif in Golgi retention for endomembrane proteins among different organisms

Molecular Biology of the Cell, v. 26, (23), November 2015, p. 4280-4293
Woo, Cheukhang; Gao, Caiji; Yu, Ping; Tu, Linna; Meng, Zhaoyue; Banfield, David Karl; Yao, Xiaoqiang; Jiang, Liwen
Article

2014 1

Cell Organelles

Banfield, David K.; Prinz, Will
Book

2012 1

A Conserved N-terminal Arginine-Motif in GOLPH3-Family Proteins Mediates Binding to Coatomer

Traffic, v. 13, (11), November 2012, p. 1496-1507
Tu, Linna; Chen, Lu; Banfield, David K.
Article

2011 1

Mechanisms of Protein Retention in the Golgi

Cold Spring Harbor Perspectives in Biology, v. 3, (8), August 2011, p. 1-14
Banfield, D.K.
Article

2010 2

Localization of Golgi-resident Glycosyltransferases

Cellular and molecular life sciences, v. 67, (1), 2010, JAN, p. 29-41
Tu, Linna; Banfield, David Karl
Article

Signal-mediated retention of Golgi-resident integral membrane proteins

Second International Symposium on Membrane Biology, Ningbo, China, 2010
Banfield, David
Conference paper

2009 2

Identification of the Sub-unit Interactions and Sorting Signals that Mediate COPI-dependent Trafficking of Yeast SNAREs

2009 Gordon Research Conference on Molecular Membrane Biology, Proctor Academy, Andover, New Hampshire (USA), 5-10 Jul 2009
Banfield, David
Conference paper

Localization of Glycosyltransferases in the Golgi.

2009 IUBMB Congress, Shanghai, PRC China (August, 2009), Shanghai, PRC China
Banfield, David K.
Conference paper

2008 5

Signal-Mediated Dynamic Retention of Glycosyltransferases in the Golgi

Science, v. 321, (5887), 2008, JUL 18, p. 404-407
Tu, Linna; Tai, William C.S.; Chen, Lu; Banfield, David K.
Article

The Golgi and ER syntaxins control anterograde and retrograde transport through the early secretory pathway

Traffic, Volume10, Pages 1629-1652
Bubeck, J.; Langhans, M.; Scheuring, D.; Foresti, O.; Banfiled, David; Denecke, J.; Robinson, D.
Article

The Syntaxins SYP31 and SYP81 Control ER–Golgi Trafficking in the Plant Secretory Pathway

Traffic, v. 9, (10), 2008, OCT, p. 1629-1652
Bubeck, Julia; Scheuring, David; Hummel, Eric; Langhans, Markus; Viotti, Corrado; Foresti, Ombretta; Denecke, Juergen; Banfield, David K.; Robinson, David G.
Article

SNAREs

The Golgi apparatus - the state of the art 110 years after Camillo's Discovery / Edited by A. Mironov and M. Pavelka. Wien : Springer, 2008, p. 43-65
Banfield, David; Hong, Wanjin
Book chapter

Characterization of a Novel Longin Domain Containing Protein from Yeast

Hunter Cellular Biology Meeting, Pokolbin, NSW, Australia
Banfield, David
Conference paper

2007 1

Signal Mediated Retention of Glycosyltransferases in the Yeast Golgi

Gordon Research Conference on Molecular Membrane Biology, Andover, New Hampshire
Banfield, David
Conference paper

2006 2

Identification of the yeast R-SNARE Nyv1p as a novel longin domain-containing protein

Molecular biology of the cell, v. 17, (10), 2006, OCT, p. 4282-4299
Wen, Wenyu; Chen, Lu; Wu, Hao; Sun, Xin; Zhang, Mingjie; Banfield, David K.
Article

Identification of the Yeast R-SNARE Nyv1p as a Novel Longin Domain-Containing Protein

46th Annual Meeting of the American Society for Cell Biology, San Diego Convention center, San Diego, California (USA), 9-13 Dec 2006
Chen, Lu; Wen, Wenyu; Wu, Hao; Sun, Xin; Zhang, Mingjie; Banfield, David K.
Conference paper

2004 4

A Novel Regulator of COPI-coated Vesicle Formation

British Society for Cell Biology Annual Spring Meeting, University of Kent at Canterbury, 31 March-3 April
Tai, William C.S.; Banfield, David K.
Conference paper

COPI-coated Vesicle Formation and SNAREs

Keystone Symposia: Golgi Apparatus and Secretory Pathway of Eukaryotic Cells, Breckenridge Colorado, USA, 20-25, January
Tai, William C.S.; Banfield, David K.
Conference paper

The Molecular Architecture of Yeast ER / Golgi SNAREs.

48th Annual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology, Banff. Alberta, Canada, March 16-20, 2005
Li, Wai Ming; Tsui, Macro; Banfield, David
Conference paper

The R-SNARE Nyv1p is sorted to the limiting membrane of the yeast cell vacuole via an AP-3-dependent YXXF signal in its longin domain.

48th Annual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology, Banff. Alberta, Canada, March 16-20, 2005
Chen, Lu; Wen, Wenyu; Wu, Hao; Zhang, Mingjie; Banfield, David K.
Conference paper

2002 1

Genes that Allow Cells to Grow in the Absence of the Golgi SNARE Sft1p: Identification of New Components of the Vesicle Budding Machinery.

European Science Foundation meeting 'EXOCYTOSIS: Membrane structure and dynamics.'
Tai, William; Banfield, David Karl
Conference paper

2001 5

An autoinhibitory mechanism for nonsyntaxin SNARE proteins revealed by the structure of Ykt6p

Science, v. 293, (5530), 2001, JUL 27, p. 698-702
Tochio, Hidehito; Tsui, M.M.K.; Banfield, David K.; Zhang, Mingjie
Article

AtBS14a and AtBS14b, two Bet1/Sft1-like SNAREs from Arabidopsis thaliana that complement mutations in the yeast SFT1 gene

FEBS letters, v. 500, (3), 2001, JUL 6, p. 177-182
Tai, WCS; Banfield, DK
Article

Selective formation of Sed5p-containing SNARE complexes is mediated by combinatorial binding interactions

Molecular biology of the cell, v. 12, (3), 2001, MAR, p. 521-538
Tsui, MMK; Tai, WCS; Banfield, DK
Article

SNARE complexes - Is there sufficient complexity for vesicle targeting specificity?

Trends in Biochemical Sciences, v. 26, (1), 2001, p. 67-68
Banfield, D.K.
Article

An Auto-Inhibitory Mechanism for Non-Syntaxin SNARE Proteins Revealed by the Structure of Ykt6p.

the Cold Spring Harbor Yeast Cell Biology Meeting
Banfield, David K.; Tochio, Hidehito; Tsui, Marco; Zhang, Mingjie
Conference paper

2000 1

Yeast Golgi SNARE interactions are promiscuous

Journal of cell science, v. 113, (1), 2000, JAN, p. 145-152
Tsui, MMK; Banfield, DK
Article

1995 2

A SNARE-like Protein Required for Traffic through the Golgi Complex

Nature, v. 375, 1995, p. 806-809
Lewis, M.J.; Pelham, H.R.B.; Banfield, David K.
Article

SNAREs involved in traffic through the Golgi Complex

Cold Spring Harbor Symposia on Quantitative Biology, v. 60, 1995, p. 105-111
Pelham, H.R.B.; Banfield, David K.; Lewis, M.J.
Article

1994 3

Characterization of a Stable Form of Human Meizothrombin Derived from Recombinant Prothrombin (R155A, R271A, R284A)

Journal of Biological Chemistry, v. 269, 1994, p. 11374-11380
Côté, H.C.F.; Stevens, W.K.; Bajzar, B.; Banfield, David K.; Nesheim, M.E.; MacGillivray, R.T.A.
Article

Evolution of Prothrombin: Isolation and Characterization of the cDNAs Encoding Chicken and Hagfish Prothrombin

Journal of Molecular Evolution, v. 38, 1994, p. 177-187
Irwin, D.M.; Walz, D.A.; MacGillivray, R.T.A.; Banfield, David K.
Article

Localisation of Sed5, a Putative Vesicle Targeting Molecule, to the cis-Golgi Network Involves both its Transmembrane and Cytoplasmic Domains

Journal of Cell Biology, v. 127, 1994, p. 357-371
Lewis, M.J.; Rabouille, C.; Warren, G.; Pelham, H.R.B.; Banfield, David K.
Article

1993 3

Characterization of Wild-Type and an Active-Site Mutant in Escherichia coli of Short-Chain Acyl-CoA Dehydrogenase from Megasphaera elsdenii

Biochemistry, v. 32, 1993, p. 10736-10742
Becker, D.F.; Fuchs, J.A.; Banfield, David K.; Funk, W.; MacGillivray, R.T.A.; Stankovich, M.T.
Article

Developmental Expression of Chicken Antithrombin III is Regulated by Increased RNA Abundance and Intracellular Processing

Biochimica et Biophysica Acta, v. 1171, 1993, p. 239-246
Amrani, D.L.; Rosenberg, J.; Samad, F.; Bergtrom, G.; Banfield, David K.
Article

Expression of Glycosylated and Non-glycosylated Human Transferrin in Mammalian Cells

Initial Characterization of the Recombinant Proteins with Comparison to Three Commercially Available Transferrin. Biochemistry, v. 32, 1993, p. 5472-5479
Mason, A.B.; Miller, M.K.; Funk, W.D.; Banfield, David K.; Savage, K.S.; Oliver, R.W.A.; Green, B.N.; MacGillivray, R.T.A.; Woodworth, R.C.
Article

1992 2

Partial Characterization of Vertebrate Prothrombin cDNAs: Amplification and Sequence Analysis of the B-Chain of Thrombin from Nine Different Species

Proceedings of the National Academy of Sciences, v. 89, 1992, p. 2779-2783
Banfield, David K.; MacGillivray, R.T.A.
Article

The Isolation and Characterization of Rabbit Motilin Precursor cDNA

Biochimica et Biophysica Acta, v. 1131, 1992, p. 341-344
MacGillivray, R.T.A.; Brown, J.C.; McIntosh, C.H.S.; Banfield, David K.
Article

1991 5

Apolipoprotein E Genotyping using the Polymerase Chain Reaction and Allele Specific Primers

Journal of Lipid Research, v. 32, 1991, p. 183-187
Main, B.F.; Jones, P.J.H.; MacGillivray, R.T.A.; Banfield, David K.
Article

Nucleotide Sequence of Rabbit Liver Transferrin cDNA

Biochimica et Biophysica Acta, v. 1089, 1991, p. 262-265
Chow, B. KC; Funk, W.; Robertson, K.; Umelas, T.; Banfield, David K.; Woodworth, R.C.; MacGillivray, R.T.A.
Article

Rapid Apolipoprotein E Genotype Identification

PCR Topics: usage of the polymerase chain reaction in genetic and infectious diseases, 1991, p. 99-102
Main, B.F.; Jones, P.J.H.; MacGillivray, R.T.A.; Banfield, David K.
Article

Structural-Functional Studies of Human Transferrin by Using In Vitro Mutagenesis

Current Studies in Heamatology and Blood Transfusion, v. 58, 1991, p. 132-138
Chow, B. KC; Funk, W.; Banfield, David K.; Lineback, J.A.; Mason, A.B.; Woodworth, R.C.; MacGillivray, R.T.A.
Article

Rapid Apolipoprotein E Genotype Identification in PCR Topics

Usage of the polymerase chain reaction in genetic and infectious diseases / Edited by Rolfs, A., Schumacher, H., and Marx, P., Berlin, Springer-Verlag, 1991, p. 99-102
Main, B.F.; Jones, P.J.H.; MacGillivray, R.T.A.; Banfield, David K.
Book chapter

1990 3

Apolipoprotein E genotyping using the polymerase chain reaction

New Zealand Medical Journal, v. 103, (884) 1990, p. 80-81
Main, Blair F.; Banfield, David K.; Jones, Peter Jh Harris
Article

Nucleotide sequence of nine protein-coding genes and 22 tRNAs in the mitochondrial DNA of the sea star Pisaster ochraceus

Institute of Molecular Biology and Biochemistry, Department of Biological Sciences, Simon Fraser University, Burnaby, V5A 1S6, British Columbia, Canada, v.31, (3), 1990, 195-204
Banfield, David K.; Smith, Michael J.; Doteval, Karin; Gorski, Sharon M.; Kowbel, David J.
Article

Protein Coding Sequences in the Sea Star Mitochondrial Genome

Journal of Molecular Evolution, v. 31, 1990, p. 195-204
Smith, M.J.; Banfield, David K.; Doteval, K.; Gorski, S.; Kowbel, D.J.
Article

1989 1

Gene Arrangement in Sea Star Mitochondrial DNA Demonstrates a Major Inversion Event During Echinoderm Evolution

Gene, v. 76, p. 181-185
Smith, M.J.; Banfield, David K.; Doteval, K.; Gorski, S.; Kowbel, D.J.
Article

1988 2

H3 Histone RNA in Eggs and Embryos of the Sea Star Piaster ochraceus

Biochemistry and Cell Biology, v. 66, 1988, p. 1040-1044
Banfield, David K.
Article

Histone Genes in Three Sea Star Species: Cluster Arrangement, Transcriptional Polarity, and Analysis of the Flanking Regions of H3 and H4 Genes

Journal of Molecular Evolution, v. 27, 1988, p. 36-44
Cool, D.; Banfield, David K.; Honda, B.M.; Smith, M.J.
Article

No Publications

Teaching Assignment

LIFS1901	General Biology I
LIFS2040	Cell Biology
LIFS3060	Microbiology
LIFS6117H	Current Topics in Cell Biology

LIFS2010	Modern Approaches to Biochemical and Cell Biological Research
LIFS4961	Biochemistry and Cell Biology Capstone Project
SCIE3500	IRE Research Project I

LIFS2040	Cell Biology
LIFS3060	Microbiology
LIFS6111T	Life Science Postgraduate Student Seminar
LIFS6117G	Current Topics in Cell Biology
SCIE2500	Guided Study on Research II

No Teaching Assignments

Research Postgraduate (RPG) Supervision

From January 2022 to December 2025 (As of 29 March 2025)

Current RPGs

Doctor of Philosophy

YE, Ziyun
Life Science
LAU, Pwu
Life Science
LESNIAK, Agnieszka Monika
Life Science

Graduated RPGs

Master of Philosophy

LI, Ruizhe
Life Science( Completed in 2024 )

Projects

From January 2023 to December 2025 (As of 29 March 2025)

No Projects.
Interrogation of a Novel Plasma Membrane Stress Signaling Pathway leading to Activation of the Spindle Assembly Checkpoint

詢問導致主軸組裝檢查點活化的新型質膜應力訊號通路 Leading

RGC - General Research Fund
Project Team (HKUST)
BANFIELD David Karl (Lead)
2024 -
Identification and delineation of the client binding sites on yGOLPH3 (Vps74p)

識別與描繪蛋白yGOLPH3 (Vps74p) 上受質蛋白質的結合位點 Leading

RGC - General Research Fund
Project Team (HKUST)
BANFIELD David Karl (Lead)
2022 - 2024
Investigation of the role of ubiquitination and de-ubiquitination in the retention of Golgi membrane proteins.

關於泛素化和去泛素化在高爾基體膜蛋白保留機制的研究 Leading

RGC - General Research Fund
Project Team (HKUST)
BANFIELD David Karl (Lead)
2020 - 2023